March 07, 2023
Petra Urgacova
Bacterium strain identification from soil sample from Lake Forest Beach, IL
Purpose
The purpose of this experiment is to determine the strain of bacterium isolated from soil samples from Lake Forest beach, IL. The bacterium is known to be antibiotically resistant as it will be taken from a McConkey agar plate with 3 μg/ml of Tetracycline concentration. We will use amplification of gene 13S ribosomal RNA which is common across prokaryotes to aid us in its identification. Sequenced data will be searched through gene databases. Techniques used include bacterium restreaking, genomic DNA isolation, Polymerase Chain Reaction (PCR), and gene sequence analysis using APE plasmid editor. We expect to find a close or identical match to a bacterium strain with known antibiotic resistance (Delventhal, 2022).